Lisa's paper describing uORF-mediated gene regulation in KSHV just published in Journal of Virology!

Reinitiation after Translation of Two Upstream Open Reading Frames Governs Expression of the ORF35-37 KSHV Polycistronic mRNA
Lisa M. Kronstad, Kevin F. Brulois, Jae U. Jung, and Britt A. Glaunsinger
J. Virol. published ahead of print 12 March 2014, doi:10.1128/JVI.00202-14


The Kaposi's sarcoma-associated herpesvirus (KSHV) ORF36 protein kinase is translated as a downstream gene from the ORF35-37 polycistronic mRNA via a unique mechanism involving short upstream open reading frames (uORFs) located in the 5’ untranslated region. Here, we confirm that ORF35-37 is functionally dicistronic during infection and demonstrate that mutation of the dominant uORF restricts KSHV replication. Leaky scanning past the uORFs facilitates ORF35 expression, while a reinitiation mechanism after the uORFs enables ORF36 translation.